Regulatory
P43

Part:BBa_K4354003:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-21)


P43 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P43 promoter is a strong constituent promoter that exists in Bacillus subtilis, which could start downstream genes transcription with a high efficiency.

Source

We synthesize it according to the NCBI‘s note(ACCESSION：EF473728).Also, a plasmid we bought has this promoter.

References

1.Song Y, Nikoloff JM, Fu G, Chen J, Li Q, Xie N, Zheng P, Sun J, Zhang D. Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications. PLoS One. 2016 Jul 5;11(7):e0158447. doi: 10.1371/journal.pone.0158447. PMID: 27380260; PMCID: PMC4933340.

2.Yu X, Xu J, Liu X, Chu X, Wang P, Tian J, Wu N, Fan Y. Identification of a highly efficient stationary phase promoter in Bacillus subtilis. Sci Rep. 2015 Dec 17;5:18405. doi: 10.1038/srep18405. PMID: 26673679; PMCID: PMC4682092.